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| Registros recuperados: 97 | |
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| Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki. |
| ABSTRACT: The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum; Tissue culture; Growth regulators. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402 |
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| PEREIRA,FERNANDO MENDES PEREIRA; USMAN,MUHAMMAD; MAYER,NEWTON ALEX; NACHTIGAL,JAIR COSTA; MAPHANGA,OSCAR RANNY MBONGENI; WILLEMSE,SALOMIE. |
| ABSTRACT Guava (Psidium guajava L.) can be propagated by seed, layering, air layering, grafting (budding or grafting), cuttings (root or shoot) or tissue culture. Propagation by seed is used for rootstock production and for raising populations for screening at early phases in the breeding programs. Vegetative propagation methods are used to clone selected genotypes from these programs and commercial orchards because it perpetuates all characteristics of each cultivar. This review addresses different methods that can be used to propagate guava, the methods commercially adopted and the progress obtained in recent years. There are several propagation technologies available, however, the adoption levels are rather different between producing countries. Needs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psidium guajava; Seeds; Grafting; Cutting; Air layering; Tissue culture. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401002 |
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| Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Junghans,Tatiana Góes; Jesus,Onildo Nunes de; Miranda,Diego; Otoni,Wagner Campos. |
| Abstract The propagation of many passion fruit species (Passiflora spp.) is done by seeds or sexual method, although asexual propagation, such stem cuttings, grafting or tissue culture is also possible and very useful in many cases. In this work, it was presented information on scientific and technological advances in sexual and asexual propagation of passion fruit, considering the different propagation methods and their applications. New demands for research and development activities are also related, given the wide range of passion fruit species and its potential for food, ornamental and medicinal use. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seed germination; Vegetative propagation; Cuttings; Grafting; Tissue culture. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000201002 |
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| MAYER,NEWTON ALEX; BIANCHI,VALMOR JOÃO; FELDBERG,NELSON PIRES; MORINI,STEFANO. |
| ABSTRACT Nursery trees of stone fruits (Prunus spp.) are traditionally produced by union of two distinct genotypes - the rootstock and the scion - which, by grafting, form a composite plant that will be maintained throughout of all plant life. In Brazil, the rootstocks are predominantly seed propagated and therefore usually results in heterogeneous trees for vigor and edaphic adaptation. However, with advances in rootstock breeding programs that released cultivars and certification in several countries (notably in Europe), the system will come gradually evolving for vegetative propagation (cuttings and tissue culture) and use of seeds of selected rootstocks with specific characteristics and potted nursery trees production. For scion cultivar propagation,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prunus spp.; Seeds; Cutting; Grafting; Air layering; Tissue culture. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401001 |
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| Sadia¹,B; Josekutty,PC; Potlakayala,SD; Patel,P; Goldman,S; Rudrabhatla,SV. |
| A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype NC+262, followed by NC+6C21 (31.3 ± 0.8), and NC+6B50 (16 ± 1.7). MS medium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Sorghum bicolor; Tissue culture; Genetic improvement; Genotypes. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200010 |
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| Takemori,N. K.; Marschner,R.; Quoirin,M.; Bona,C.; Zanette,F.. |
| In order to achieve adventitious shoot regeneration from young tissues of Racosperma mangium, several combinations of growth regulators were tested. Kinetin (KIN) and a -naphthalenacetic acid (NAA), thidiazuron (TDZ) and NAA or indole-3-butyric acid (IBA), 4-CPPU and 2,4-dichlorophenoxyacetic acid (2,4-D) were added to basic medium composed of MS salts, MS vitamins and 3% sucrose. Sections of hypocotyls and cotyledons of young seedlings were cultivated on these media. Calli developed on different media and, after two to three months, some of them regenerated organized structures. Histological studies revealed hypertrophy and hyperplasy of medular and cortical parenchyma cells of hypocotyl and cotyledon parenchyma. Formation of meristematic cells and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 4-CPPU; Leguminosae; TDZ; Tissue culture. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100008 |
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| Rodrigues,Antonio Anderson de Jesus; Santos,Eder de Oliveira; Takane,Roberto Jun; Carvalho,Ana Cristina Portugal Pinto de. |
| ABSTRACT The technique of etiolation is widely used in the micropropagation of various plant species. The aim of this study was to evaluate growth and development in shoots of Cattleya labiata grown in vitro, as a function of growth regulators and the presence and absence of light. Stem segments, approximately ± 1.0 cm in height, were inoculated in test tubes containing 15.0 mL of MS culture medium supplemented with different concentrations of BAP (0.0, 2.0 and 4.0 mg L-1) and NAA (0.0, 1.0 and 2.0 mg L-1), and in different growth environments (growth room, darkness and a photoperiod of 16 hours) in a completely randomised design. At 150 days, the following were evaluated: a) number of etiolated shoots; b) number of nodes per etiolated shoot; c) length of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchidaceae; Tissue culture; Photoperiod; 6-benzylaminupurine; Naphthaleneacetic acid. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000200296 |
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| Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz. |
| Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Organogenesis; Anatomy. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013 |
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| Lima,Ednabel Caracas; Paiva,Renato; Nogueira,Raírys Cravo; Soares,Fernanda Pereira; Emrich,Eduardo Bucsam; Silva,Álvaro Augusto Naves. |
| Croton urucurana Baill., a species belonging to the family Euphorbiaceae, can be useful in the recovery of degraded riparian areas. In the traditional medicine, it is widely used as cicatrizant and in the treatment of rheumatism. However, its seeds present dormancy and low viability, making the propagation of this species a challenge. With the objective of establishing an alternative route for the propagation, the effect of different concentrations of 2,4-D (2,4- dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) or TDZ (thidiazuron) and of NAA (1-naphthaleneacetic acid) with BAP and their possible combinations were evaluated for callus induction in leaf segments. Callus fresh mass was evaluated forty-five days after inoculation. The isolated use... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Calogenesis; Tissue culture. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100002 |
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| Martínez Hernández, María de Jesús. |
| La multiplicación de plantas in vitro presenta varias etapas críticas, desde la selección de los componentes para la germinación, multiplicación y enraizamiento hasta el proceso de aclimatación en invernadero. En la presente investigación se utilizaron los sustratos inertes (vermiculita, agrolita y tezontle) como medio de soporte en sustitución del agar y los azúcares xilosa, fructosa y 10X20F se compararon con la sacarosa, en su efecto sobre la germinación, multiplicación, enraizamiento y sobrevivencia de los portainjertos de cítricos: Citrus volkameriana, Citrumelo swingle y Citrange 35. Además de probar diferentes intensidades luz en el proceso de aclimatación de plántulas de Citrus volkameriana con diferentes sustratos y una reducción gradual... |
| Tipo: Tesis |
Palavras-chave: Cultivo in vitro; Sustratos inertes; Cítricos; Doctorado; Agroecosistemas tropicales; Tissue culture; Inert substrates; Citrus. |
| Ano: 2007 |
URL: http://hdl.handle.net/10521/1263 |
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| Sá,Jucieny Ferreira de; Sampaio,Emília dos Santos; Mendes,Maria Inês de Souza; Santos,Karen Cristina Fialho dos; Sousa,Antônio da Silva; Ledo,Carlos Alberto da Silva. |
| ABSTRACT The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cassava; Wild parental; Nutritional media; Tissue culture; Micropropagation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600598 |
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| Silva,Luciano Coutinho; Paiva,Renato; Vargas,Daiane Peixoto; Silva,Diogo Pedrosa Corrêa da; Barbosa,Sandro; Herrera,Raírys Cravo. |
| Byrsonima intermedia A. Juss. é uma planta medicinal e frutífera do Cerrado, cuja propagação convencional é difícil, devido à presença do endocarpo extremamente lignificado. Embora o hipoclorito de sódio (NaOCl) seja amplamente utilizado para a desinfestação superficial, existem poucos relatos de seus efeitos sobre o crescimento do explante. Neste trabalho, o objetivo foi estudar o efeito de diferentes pHs e períodos de exposição de sementes de B. intermedia em uma solução de NaOCl. As sementes foram submetidas a diferentes tempos de exposição (1, 5 e 10 minutos) numa solução de NaOCl com diferentes pHs (5, 7, 10 e 12) e, após o tratamento com hipoclorito de sódio, os embriões foram inoculados em meio WPM com a concentração de 50% de sais sem sacarose,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micropropagation; Native plant; Tissue culture; PH; NaOCl.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400674 |
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| Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de. |
| This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo culture; Tissue culture; Biotechnology; Citrus breeding. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031 |
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| Registros recuperados: 97 | |
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